Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on VEGFR2 protein expression in rat vascular endothelial cells. ( A ) Western blot bands of indicating proteins in endothelial cells; ( B ) densitometric analysis of the ratio of the intensity of protein bands. Values are the mean ± SD of VEGFR2 expression standardized to β-actin expression in three independent experiments; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Western Blot

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on proliferation and migration of endothelial cells. ( A ) BrdU-positive cell rate; ( B ) migration distance statistics chart. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Migration

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on tube formation of endothelial cells. ( A ) Number of nodes forming a tube-like structure; ( B ) branch lengths forming the tube-like structure. Data expressed as mean ± SD; ** p < 0.01, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the mouse matrigel plug model. ( A ) Pictures of the mouse matrigel plugs (a: PBS group, b: NC group, c: ROX group, d: sh-Vegfr2 group, and e: sh-Vegfr2 + ROX group); ( B ) hemoglobin content in matrigel plugs. Data expressed as mean ± SD; * p < 0.05, ** p < 0.01, compared with the PBS group; # p < 0.05, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques:

Journal: Veterinary Sciences

Article Title: A Recombinant Lentiviral Vegfr2-Silencing Vector Attenuates Roxarsone-Promoted Growth of Rat Vascular Endothelial Cells and Angiogenesis in Matrigel Plug and B16F10 Xenograft Models

doi: 10.3390/vetsci11100451

Figure Lengend Snippet: Effects of ROX and/or sh-Vegfr2 on the growth and angiogenesis of/in melanoma xenografts. ( A ) Melanoma xenograft tumor volumes during the test period; ( B ) melanoma xenograft tumor weight at necropsy; ( C ) immunohistochemical expression of CD34 in melanoma xenografts. Data expressed as mean ± SD; * p < 0.05, compared with the PBS group; ## p < 0.01, compared with the ROX group.

Article Snippet: Rabbit anti-rat VEGFR2 polyclonal antibody, mouse anti-rat β-actin monoclonal antibody, HRP-labeled goat anti-rabbit IgG, HRP-labeled goat anti-mouse IgG and SABC-POD three-step detection kit were purchased from Boster Biological Technology Co., Ltd. (Wuhan, China).

Techniques: Immunohistochemical staining, Expressing

Journal: Translational Vision Science & Technology

Article Title: Cytokine Levels in Experimental Branch Retinal Vein Occlusion Treated With Either Bevacizumab or Triamcinolone Acetonide

doi: 10.1167/tvst.13.6.13

Figure Lengend Snippet: Graph of RT-qPCR (normalized against the housekeeping gene GAPDH ) showing the regulatory effect of BRVO on the mean value of the expression of pig retinal genes. ( A ) Highly elevated levels in IL-8 , GFAP , AQP4 , CCL2 , ICAM 1, and HSF1 . ( B ) Moderately upregulated genes VEGFA , KCNJ1 0 , and CXCL12 . ( C ) Non-significant changes in IL 6 and DMD at 2, 6,10, and 20 days post-occlusion. Significant difference are indicated by a black asterisk (*).

Article Snippet: Briefly, the sections were blocked with serum followed by incubation with the primary antibody polyclonal rabbit anti-human VEGFA (Proteintech, Rosemont, IL) at 1:150 dilution.

Techniques: Quantitative RT-PCR, Expressing

Journal: Translational Vision Science & Technology

Article Title: Cytokine Levels in Experimental Branch Retinal Vein Occlusion Treated With Either Bevacizumab or Triamcinolone Acetonide

doi: 10.1167/tvst.13.6.13

Figure Lengend Snippet: Graph of RT-qPCR (normalized against the housekeeping gene GAPDH ) showing the mean value of the expression of VEGFA ( A ) and KCNJ10 ( B ) at the same time point, where # indicates a significant difference from untreated BRVO, $ indicates a significant difference from TA, and * indicates a significant difference from healthy controls.

Article Snippet: Briefly, the sections were blocked with serum followed by incubation with the primary antibody polyclonal rabbit anti-human VEGFA (Proteintech, Rosemont, IL) at 1:150 dilution.

Techniques: Quantitative RT-PCR, Expressing